ZFYVE1抗体—艾普蒂

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Mouse kidney tissue lysate, Primary antibody: P09221(ZFYVE1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P09221(ZFYVE1 Antibody) at dilution 1/90. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P09221(ZFYVE1 Antibody) at dilution 1/90. (Original magnification: ×200)    

  
  

ZFYVE1 antibody is a research tool targeting the ZFYVE1 protein, encoded by the ZFYVE1 gene (zinc finger FYVE-type containing 1), also known as DFCP1 (Double FYVE-containing protein 1). ZFYVE1 is a membrane-associated protein involved in intracellular vesicle trafficking, autophagy, and endosomal dynamics. It contains a FYVE domain, which binds phosphatidylinositol 3-phosphate (PI3P), enabling its localization to membranes of the endoplasmic reticulum (ER), Golgi apparatus, and omegasomes—transient structures critical for autophagosome formation.

The ZFYVE1 protein plays a regulatory role in autophagy by interacting with autophagy-related proteins like ULK1 and WIPI2. facilitating membrane remodeling during autophagosome biogenesis. It is also implicated in maintaining ER morphology and lipid droplet dynamics. Antibodies against ZFYVE1 are widely used in immunoblotting (WB), immunofluorescence (IF), and immunoprecipitation (IP) to study its expression, subcellular localization, and molecular interactions.

Research utilizing ZFYVE1 antibodies has provided insights into diseases linked to autophagy dysregulation, including neurodegenerative disorders, cancer, and metabolic syndromes. Variations in ZFYVE1 expression or function have been observed in certain cancers, suggesting potential roles in tumor progression or suppression. Commercial ZFYVE1 antibodies are typically validated for specificity across human, mouse, and rat samples, with applications spanning basic cell biology and translational studies. Proper controls, such as knockout cell lines, are recommended to confirm antibody specificity in experimental settings.