SNF8抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P09212(SNF8 Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

The SNF8 antibody is a research tool designed to detect and study the SNF8 protein, a critical component of the endosomal sorting complex required for transport (ESCRT) machinery. SNF8 (also known as VPS22) is part of the ESCRT-II complex, which collaborates with ESCRT-I and ESCRT-III to mediate membrane remodeling processes, including multivesicular body (MVB) formation, cytokinesis, and viral budding. SNF8 facilitates the sorting of ubiquitinated cargo proteins into MVBs for lysosomal degradation, a process essential for cellular homeostasis, receptor downregulation, and signal transduction. Dysregulation of SNF8 has been linked to cancer, neurodegenerative disorders, and immune dysfunction, underscoring its biological significance.

The SNF8 antibody is typically developed using immunogens such as recombinant SNF8 protein fragments or synthetic peptides. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess SNF8 expression, localization, and interactions in cell lines or tissues. Validation often involves knockout or knockdown models to confirm specificity. Researchers employ this antibody to explore ESCRT-dependent pathways, investigate disease mechanisms, or study viral pathogens (e.g., HIV-1) that hijack ESCRT components for replication. Its utility spans molecular biology, cancer research, and virology, making it a vital reagent for understanding membrane trafficking and related pathologies.