RPL8抗体—艾普蒂

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     Western blot analysis using GSTP1 mouse mAb against PC3 cell lysate (1) and human cerebellum tissue lysate (2).    

  
  

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     Confocal Immunofluorescence analysis of HepG2 (left) and L-02 (right) cells using GSTP1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.    

  
  

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     Confocal Immunofluorescence analysis of PC-3 cells using GSTP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.    

  
  

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     Flow cytometric analysis of K562 cells using GSTP1 mouse mAb (green) and negative control (purple).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human prostate tissues using GSTP1 mouse mAb with DAB staining.    

  
  

The RPL8 antibody is a tool used to detect ribosomal protein L8 (RPL8), a component of the 60S ribosomal subunit involved in protein synthesis. RPL8. encoded by the RPL8 gene in humans, plays a structural role in ribosome assembly and facilitates mRNA binding during translation. Antibodies targeting RPL8 are widely utilized in research to study ribosomal biogenesis, cellular stress responses, and diseases linked to ribosomal dysfunction, such as cancer and genetic disorders like Diamond-Blackfan anemia.

In cancer biology, RPL8 expression is often dysregulated, and its detection helps assess tumor progression or therapy resistance. The antibody is employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to localize and quantify RPL8 in tissues or cell lines. It also aids in investigating post-translational modifications of RPL8 under stress conditions, such as nutrient deprivation or oxidative stress.

Commercially available RPL8 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins as immunogens. Validation includes testing for specificity via knockout cells or siRNA-mediated silencing. Researchers must optimize conditions (e.g., dilution, fixation) to minimize cross-reactivity with homologous ribosomal proteins. Overall, the RPL8 antibody serves as a critical reagent for exploring ribosome-related mechanisms in both basic and translational research contexts.