RNF141抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P09184(RNF141 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P09184(RNF141 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

The RNF141 antibody is a research tool designed to detect and analyze the Ring Finger Protein 141 (RNF141), an E3 ubiquitin ligase encoded by the *RNF141* gene in humans. RNF141 belongs to the RING finger protein family, characterized by a conserved zinc-binding RING domain critical for mediating ubiquitin transfer during protein ubiquitination. This post-translational modification regulates diverse cellular processes, including protein degradation, DNA repair, and signal transduction. RNF141 has been implicated in germ cell development, spermatogenesis, and tumorigenesis, with studies suggesting roles in modulating apoptosis, cell cycle progression, and transcriptional regulation.

Antibodies targeting RNF141 are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They enable researchers to investigate RNF141 expression, localization, and interaction partners via techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Specificity is validated through controls like knockout cell lines or siRNA-mediated knockdown.

RNF141 antibodies are particularly valuable in reproductive biology and oncology research, as aberrant RNF141 expression has been linked to infertility and cancers such as hepatocellular carcinoma. Understanding RNF141's molecular mechanisms may provide insights into disease pathways and therapeutic targets. Researchers prioritize antibodies with high affinity, minimal cross-reactivity, and compatibility with their experimental models to ensure reliable data generation.