NDUFA12抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human fetal muscle tissue, PC-3 and Jurkat cell, Primary antibody: P09135(NDUFA12 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P09135(NDUFA12 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P09135(NDUFA12 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The NDUFA12 antibody is a research tool designed to detect and study the NDUFA12 protein, a critical subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) within the electron transport chain. Complex I facilitates ATP production by transferring electrons from NADH to ubiquinone, coupled with proton pumping across the mitochondrial inner membrane. NDUFA12. encoded by the *NDUFA12* gene in humans, contributes to the structural stability and catalytic activity of Complex I. Dysfunction in Complex I subunits, including NDUFA12. is linked to mitochondrial disorders, neurodegenerative diseases, and cancer, making this protein a focus of metabolic and pathological research.

NDUFA12 antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and abundance in tissues or cultured cells. These antibodies help investigate how NDUFA12 deficiencies or mutations impair mitochondrial respiration, ROS regulation, or cellular energy homeostasis. Studies involving NDUFA12 antibodies have provided insights into diseases such as Leigh syndrome, Leber hereditary optic neuropathy (LHON), and certain cancers, where altered Complex I activity is observed. Additionally, they aid in exploring therapeutic strategies targeting mitochondrial dysfunction. Validation of antibody specificity via knockout controls or siRNA knockdown is essential to ensure reliable results. Overall, NDUFA12 antibodies serve as vital tools in both basic and translational research focused on mitochondrial biology and associated diseases.