Recombinant Human IL-27(His Tag)重组人白介素-27

同用名/别名（Synonym）

Interleukin-27 subunit alpha; IL-27 subunit alpha; IL-27-A; IL27-A; p28; Il27; Il27a; Interleukin-27 subunit beta; Ebi3; IL-27 subunit beta; IL-27B; Epstein-Barr virus-induced gene 3 protein homolog; Il27b

描述（Description）

IL-27 (Interleukin-27) is a heterodimeric cytokine of the IL-12 family, composed of two subunits, EBI3 (Epstein-Barr-virus-induced molecule 3) and p28^[1]. IL-27 enhances the development, proliferation, and cytotoxic activity of CD8⁺ T cells, thereby indirectly promoting anti-tumor immunity^{[ 2]}. IL-27 Protein, Human (CHO, His) is a recombinant protein with a His label that consists of two subunits, EBI3 (IL27B) (229 amino acids (M1-K229)) and IL27A (214 amino acids (F29-P243)), is produced by CHO cells.

纯度（Purity）

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

生物活性（Bioactivity）

1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/mL (100 μl/well) can bind human IL27RA-Fc, The EC₅₀ of human IL27RA-Fc is 0.2-0.46 μg/mL.
2. Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus(VSV). The ED50 for this effect is typically ≤92 ng/mL.

氨基酸序列（Target Protein Sequence）

RKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK

制剂（Trait）

Lyophilized from a 0.2 μm filtered solution of 20 mM MOPS,150 mM NaCl, 0.05% CHAPS, PH7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

复溶（Reconstitution）

It is recommended to redissolve in 4mM HCl.

保存条件&效期（Storage & Stability）

Stored at -20°C for 1 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.Please avoid repeated freeze-thaw cycles.

运输方式(Shipping)

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

生物活性（Bioactivity）

Measured in a VSV inhibition assay with Wish cells. The ED50 is ≤1 ng/ml.

特别提示：本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。