Recombinant Human G-CSF 重组人粒细胞集落刺激因子

描述（Description）

Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein secreted by the cells of the immune system, fibroblasts and endothelium, which acts as a hematopoietic and endothelial precursor cells cytokine. G-CSF stimulates maturation of progenitor cells in the bone marrow into differentiated granulocytes, macrophages and the T cells. G-CSF also shows to convey neuroprotection to central neurons upon increases in phosphorylation of PI3K/Akt pathway and regulates epithelial to mesenchymal transition in cancer. G-CSF Protein (Human) is a recombinant protein with tag free that consists of 200 or 204 amino acids.

生物活性（Bioactivity）

The ED50 is <0.1 ng/mL as measured by M-NFS-60 cells, corresponding to a specific activity of >1.0 × 107 units/mg.

氨基酸序列（Target Protein Sequence）

TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

制剂（Trait）

Lyophilized from a 0.2 μm filtered solution of 10 mM HAc-NaAc, 150 mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0 or 20 mM PB, 150 mM NaCl, pH 7.4 or 25 mM Tris, pH 8.0 or 10mM HAc-NaAc, 150 mM NaCl, pH 4.0 or 10mM HAc-NaAc, 150 mM NaCl, pH 4.0, 10% trehalose, 0.02% Tween80 or PBS, pH 7.4, 8% trehalose.

复溶（Reconstitution）

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件&效期（Storage & Stability）

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输方式(Shipping)

Room temperature in continental US; may vary elsewhere.

生物活性（Bioactivity）

The activity is determined by the dose-dependent proliferation of murine NFS-60 cells is ＜ 0.5 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

电泳（SDS-PAGE）

Greater than 95% as determined by reducing SDS-PAGE.

特别提示：本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。