Recombinant Human Fibronectin-GMP重组人纤连蛋白

描述（Description）

Fibronectin binds collagen, fibrin, heparin, DNA, and actin. It is involved in cell adhesion, motility, opsonization, wound healing and maintenance of cell shape. GMP Fibronectin Protein, Human is a recombinant protein dimer complex containing human-derived Fibronectin protein, expressed by E. coli , with tag free. GMP Fibronectin Protein, Human, has molecular weight of ~60.0 kDa.

生物活性（Bioactivity）

1.Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is ≤ 385.7 ng/mL.

2.Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is ≤182.9 ng/mL, corresponding to a specific activity is ≥5.467×103 units/mg.

氨基酸序列（Target Protein Sequence）

PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPS&AIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST

制剂（Trait）

Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 5.5 or PBS, pH 6.8, 5% trehalose, 5% mannitol and 0.01% Tween 80.

复溶（Reconstitution）

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件&效期（Storage & Stability）

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输方式(Shipping)

Room temperature in continental US; may vary elsewhere.

生物活性（Bioactivity）

Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 124.5 ng/mL, corresponding to a specific activity is 8.03×103 units/mg.

电泳（SDS-PAGE）

Greater than 95% as determined by reducing SDS-PAGE.

特别提示：本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。