Recombinant Human TGF-β1重组人转化生长因子-β1

描述（Description）

TGF beta 1/TGFB1 Protein is initially identified as a growth factor that induces the growth of rodent fibroblasts. TGF beta 1/TGFB1 Protein inhibits the cell cycle in the G1 phase. TGF beta 1/TGFB1 is an endogenous factor controlling apoptosis in normal and pathological tissues. TGF beta 1/TGFB1 Protein, Human is a recombinant protein (A279-S390) produced by CHO cells.

生物活性（Bioactivity）

1. The ED50 is <0.2 ng/mL as measured in ability to inhibit the mouse IL-4-dependent proliferation of HT-2 cells.
2. The ability to inhibit the IL-4-dependent proliferation of TF 1 human erythroleukemic cells has an ED50 value of 4-40 pg/mL.
3. Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is ≤0.09738 ng/mL, corresponding to a specific activity is ≥1.027×107 units/mg.

氨基酸序列（Target Protein Sequence）

ALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

制剂（Trait）

Lyophilized from a 0.2 μm filtered solution of 50 mM NaAc, 50 mM NaCl, pH 5.0 or 50 mM Glycine-HCl, 150 mM NaCl, pH 2.5 or 4 mM HCL.

复溶（Reconstitution）

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or diluted with 50 mM Citrate. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件&效期（Storage & Stability）

Stored at -20°C for 1 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.Please avoid repeated freeze-thaw cycles.

运输方式(Shipping)

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

生物活性（Bioactivity）

Measured in a cell inhibition assay using TF-1 cells at IL-4 presence. The ED50 for this effect is ≤0.1ng/mL. The Bioactivity of F1d0058was higher than other competing products.

电泳（SDS-PAGE）

Recombinant Human TGF-β1 (Cat. No. F1d0058)  SDS-PAGE under reducing (R) & Non-reducing condition. The gel was stained withF1d0058

特别提示：本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。