Recombinant Human PDGF-BB重组人血小板衍生生长因子-BB

描述（Description）

Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.

氨基酸序列（Target Protein Sequence）

SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT

制剂（Trait）

Lyophilized after extensive dialysis against PBS, pH 7.4 or 20 mM NaAc-HAc, pH 4.5 or 50 mM Tris-HCL, 200 mM NaCl, 500 mM arginine, pH 8.0.

复溶（Reconstitution）

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件&效期（Storage & Stability）

Stored at -20°C for 1 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.Please avoid repeated freeze-thaw cycles.

运输方式(Shipping)

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

生物活性（Bioactivity）

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤5 ng/mL. 

The bioactivity of Y03401 is equivalent to the other competing product.      

电泳（SDS-PAGE）

Recombinant Human PDGF-BB (Cat. No. F1d0054) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was 

stained with Y03401 SDS-PAGE.

特别提示：本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。