6769-80-8 5-溴-6-氯-3-吲哚磷酸酯，对甲苯胺盐

5-溴-6-氯-3-茚氧基磷酸对甲苯胺盐，也称为品红色-磷对甲苯胺盐，是一种用于观察碱性磷酸酶活性区域的组织化学物质。碱性磷酸酶水解5-溴-6-氯-3-吲哚氧基磷酸上的磷酸基团，生成不溶的靛蓝化合物。可使相关化合物的磷酸盐水解产生的吲哚类物质与四唑鎓盐反应，从而将四唑鎓还原为彩色的不溶性甲瓒。相关物质5-溴-4-氯-3-吲哚磷酸盐的不溶水解产物的增溶技术允许在定量ELISA实验中使用该底物。5-溴-6-氯-3-吲哚磷酸酯，对甲苯胺盐是TNAP的底物。

5-Bromo-6-chloro-3-indoxyl phosphate p-toluidine salt, also known as Magenta-phos p-toluidine salt, is a histochemical substance used for visualizing areas of alkaline phosphatase activity. Hydrolysis of the phosphate group on 5-Bromo-6-chloro-3-indoxyl phosphate by alkaline phosphatase generates an insoluble indigo compound. The indoxyl species resulting from phosphate hydrolysis of related compounds can be reacted with tetrazolium salts, reducing the tetrazolium to a colorful insoluble formazan species. A solubilization technique involving reduction of the insoluble hydrolytic product of the closely related 5-Bromo-4-chloro-3-indoxyl phosphate permitted the use of this substrate in quantitative ELISA experiments. 5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt is a substrate of TNAP.