化合物 PP121|T2415|TargetMol

PP121
1092788-83-4

￥197 1mg 起订

￥431 5mg 起订

￥693 10mg 起订

上海更新日期：2025-11-17

TargetMol中国（陶术生物）

VIP4年

联系人：邵小姐

手机：4008200310 拨打

邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: 化合物 PP121

英文名称：: PP121

CAS号：: 1092788-83-4

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.67%

产品类别：: 抑制剂

货号：: T2415

Product Introduction

Bioactivity

名称	PP121
描述	PP-121 is a multi-targeted inhibitor of PDGFR (IC50: 2 nM), Hck (IC50: 8 nM), mTOR (IC50: 10 nM), VEGFR2(IC50: 12 nM), Src (IC50: 14 nM) and Abl (IC50: 18 nM) , also inhibits DNA-PK (IC50: 60 nM).
细胞实验	For western blot analysis, cells are grown in 12-well plates and treated with PP-121 at the indicated concentrations or vehicle (0.1% DMSO). Treated cells are lysed, lysates are resolved by SDS-PAGE, transferred to nitrocellulose and blotted. For cell proliferation assays,cells are grown in 96-well plates are treated with PP-121 at 4-fold dilutions (10 μM - 0.040 μM) or vehicle (0.1% DMSO). After 72 hours cells are exposed to Resazurin sodium salt (22 μM) and fluorescence is quantified. IC50 values are calculated using Prism software. For proliferation assays involving single cell counting, non-adherent cells are plated at low density (3–5% confluence) and treated with PP-121 (2.5 μM) or vehicle (0.1% DMSO). Cells are diluted into trypan blue daily and viable cells counted using a hemocytometer. For apoptosis and cell cycle analysis, cells are treated with the indicated concentration of PP-121 or vehicle (0.1% DMSO) for 24–72 hours. Cells are either stained live with AnnexinV-FITC or fixed with ethanol and stained with propidium iodide. Cell populations are separated using a FacsCalibur flow cytometer; data is collected using CellQuest Pro software and analyzed with either ModFit or FlowJo Software.(Only for Reference)
激酶实验	Kinase assays: Purified kinase domains are incubated with PP-121 at 2- or 4-fold dilutions over a concentration range of 1 nM-50 μM or with vehicle (0.1% DMSO) in the presence of 10 μM ATP, 2.5 μCi of γ-32P-ATP and substrate. Reactions are terminated by spotting onto nitrocellulose or phosphocellulose membranes, depending on the substrate; this membrane is then washed 5–6 times to remove unbound radioactivity and dried. Transferred radioactivity is quantitated by phosphorimaging and IC50 values are calculated by fitting the data to a sigmoidal doseresponse using Prism software.
体内活性	作用于胶质瘤细胞系（U87和LN229）时,PP-121（0.04-10 μM）可剂量依赖性地有效抑制Akt、p70S6K 和S6磷酸化。PP-121（0.04-10 μM）通过直接抑制PI3Ks 和 mTOR,而有效抑制一系列肿瘤细胞增殖。作用于LN220,U87和Seg1细胞时,PP-121可诱导细胞在G0/G1期停滞。作用于转化v-Src(Thr338)的NIH3T3 细胞,PP-121（0.08-20 μM）抑制v-Src诱导的酪氨酸磷酸化。作用于转化v-Src(Thr338)的NIH3T3细胞时,PP-121（2.5 μM）使肌动蛋白纤维的染色恢复。作用于表达C634W致癌基因 Ret突变35的TT甲状腺癌细胞时,PP-121（40 nM）抑制Ret自磷酸化。PP-121抑制 TT 甲状腺癌细胞增殖（IC50：50 nM）。作用于人脐静脉内皮细胞时,PP-121抑制VEGF刺激的细胞增殖（IC50：41 nM）。
存储条件	keep away from moisture,keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : < 1 mg/mL (insoluble or slightly soluble) Ethanol : 2 mg/mL (6.26 mM), Sonication is recommended. DMSO : 60 mg/mL (187.88 mM), Sonication is recommended.
关键字	VEGFR \| Vascular endothelial growth factor receptor \| Src \| PP-121 \| PP121 \| PP 121 \| Platelet-derived growth factor receptor \| PDGFR \| mTOR \| Mammalian target of Rapamycin \| Inhibitor \| inhibit \| Hck \| BcrAbl \| Apoptosis \| Abl
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PP121|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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