Anti IDH2(R172G) Mouse Monoclonal Antibody

IDH2(R172G) [100ul] Catalog Number:  CR-M26231 Gene Symbol:  IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH Description:   Anti IDH2(R172G) Mouse Monoclonal Antibody Background:   Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172G IDH2 mutations do exist in diffusely infiltrative gliomas. Immunogen:   A synthetic peptide from the internal region of IDH2 which includes the mutation of R172G, human origin. Applications:   ELISA, WB, IHC Recommended Dilutions:    ELISA: 1:1000-1:5000  WB: 1:100-1:1000  IHC: 1:50-1:100 Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:    Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  recognizes R172G mutant, but not wild-type IDH2 of vertebrates. Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles Western blot:

Western blot analysis of recombinant IDH2(R172G) and wildtype proteins. Purified His-tagged IDH2(R172G) (lane 1) and corresponding wild-type IDH2 protein (lane 2) were blotted with Anti IDH2(R172G) monoclonal antibody (Cat. #CR-M26231).

Immunofluorescence:

Immunofluorescence of cells expressing IDH2 proteins with Anti IDH2(R172G) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172G) plasmid (right column), then fixed and stained with Anti IDH2(R172G) monoclonal antibody (Cat. #CR-M26231).