Anti IDH1(R132H) Mouse Monoclonal Antibody

DH1(R132H)  [100 µL] Catalog Number:  CR-M26081 Gene Symbol:  IDH1 Description:   Anti IDH1(R132H) Mouse Monoclonal Antibody Background:   Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. Immunogen:   Recombinant protein of human IDH1 which includes the mutation of R132H. Applications:   ELISA, WB, IHC, IP Recommended Dilutions:     ELISA: 1:1000-1:5000  WB: 1:100-1:1000  IHC: 1:50-1:100 Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Western blot:

Western blot analysis of recombinant IDH1(R132H) and wild-type proteins. Purified His-tagged IDH1(R132H) protein (Cat.#CR-P10199) and wild-type protein (amino acid 1-240) (Cat.#CR-P10198) were blotted with anti IDH1(R132H) mouse antibody (Cat.#CR-M26081)(Left panel) or with anti IDH1 mouse antibody (Cat. #CR-M26136 right panel).

Immunofluorescence:

Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1(R132H) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH1(WT) plasmid (left column) or pCDNA3-GFP IDH1(R132H) plasmid (right column), then fixed and stained with Anti IDH1(R132H) monoclonal antibody (Cat. #CR-M26081).