Anti Ras(G13D) Mouse Monoclonal Antibody

Ras(G13D)  [100 µL] Catalog Number:  CR-M26038 Gene Symbol:  Ras(G13D) Description:   Anti Ras(G13D) Mouse Monoclonal Antibody Background:   Ras(G13D) is one type of Ras missense mutations at the codon 13. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G13D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. Immunogen:    A synthetic peptide from the internal region of Ras which includes the mutation of G13D, human origin. Applications:   ELISA, WB, IF, IHC Recommended Dilutions:    ELISA: 1:100-1:3000  WB: 1:200-1:1000  IF: 1:50-1:100  IHC: 1:50-1:100 Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG2b Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  RasG13D antibody recognizes RasG13D proteins of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Western blot:

Western blot analysis of recombinant Ras WT and G13D proteins with anti Ras(G13D) monoclonal antibody. Equal amounts of purified Ras WT (lane 1, Cat # CR-P10172) and G13D (lane 2, Cat # CR-P10155) proteins were loaded and blotted with anti Ras(G13D) mouse monoclonal antibody (Cat #CR-M26038) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti Ras antibody (Cat # CR-R21021).

Immunofluorescence:

Immunofluorescence of cells expressing RAS proteins with Anti Ras(G13D) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G13D) plasmid,pCDNA3-GFP Ras(WT)plasmid, pCDNA3-GFP Ras(G13A) plasmid,pCDNA3-GFP Ras(G13V) plasmid, pCDNA3-GFP Ras(G13C)plasmid, pCDNA3-GFP Ras(G12R)plasmid or pCDNA3-GFP Ras(G13S)plasmid, then fixed and stained with anti-Ras(G13D) monoclonal antibody (Cat. # CR-M26038).