Anti active Arl3 Mouse Monoclonal Antibody

Active Arl3 GTP  [30 µL] Catalog Number:  CR-M26925 Gene Symbol:  ARL3 Description:   Anti active Arl3 Mouse Monoclonal Antibody Background:   Arl3 (Arf-like 3) is an Arf family protein. Arl3 binds microtubules to alter specific aspects of cytokinesis. It has been proposed that retinitis pigmentosa 2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery. Immunogen:   Recombinant Arl3 protein (active form) Applications:   IP, IHC, IF Recommended Dilutions:    1 µg for 1~2 mg total cellular proteins for IP  1:50~100 dilution for IHC, IF Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Arl3 antibody recognizes active Arl3-GTP from vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Immunoprecipitation/Western blot:   Arl3 Activation Assay. Purified His-tagged Arl3 proteins (Cat. #CR-P10152) were immunoprecipitated with the anti active Arl3 monoclonal antibody (Cat. #CR-M26925) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti Arl3 monoclonal antibody (Cat. #CR-R21070). Input controls are shown in the bottom panel.Active Arl3 GTP  [30 µL] Catalog Number:  CR-M26925 Gene Symbol:  ARL3 Description:   Anti active Arl3 Mouse Monoclonal Antibody Background:   Arl3 (Arf-like 3) is an Arf family protein. Arl3 binds microtubules to alter specific aspects of cytokinesis. It has been proposed that retinitis pigmentosa 2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery. Immunogen:   Recombinant Arl3 protein (active form) Applications:   IP, IHC, IF Recommended Dilutions:    1 µg for 1~2 mg total cellular proteins for IP  1:50~100 dilution for IHC, IF Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Arl3 antibody recognizes active Arl3-GTP from vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Immunoprecipitation/Western blot:

Arl3 Activation Assay. Purified His-tagged Arl3 proteins (Cat. #CR-P10152) were immunoprecipitated with the anti active Arl3 monoclonal antibody (Cat. #CR-M26925) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti Arl3 monoclonal antibody (Cat. #CR-R21070). Input controls are shown in the bottom panel.