Anti Active Gαi Mouse Monoclonal Antibody

Active Gαi GTP  [30 µL] Catalog Number:  CR-M26901 Gene Symbol:  Gnai Description:   Anti Active Gαi Mouse Monoclonal Antibody Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαirepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαi proteins are being explored. Immunogen:   Recombinant full length protein of active Gαi1 Applications:   IP, IHC Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;                                                     1:100 dilution for IHC Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG1 Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti active Gαi antibody recognizes active Gαi1, Gαi2, and Gαi3 of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Gαi activation assay.  A. CHO cells were transfected with wild-type Gαi1 (lanes 1 and 2) or constitutively active Gαi1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gαi monoclonal antibody (Cat. No.26901) (top panel). The precipitated active Gαi was immunoblotted with  an anti Gαi monoclonal antibody (Cat. No. CR-M26003). The bottom panel shows the Western blot with anti Gαi monoclonal antibody (Cat.No. CR-M26003) of the cell lysates.  B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or  without (lane 1)carbachol. Cell lysates were then incubated with an anti active Gαi monoclonal antibody  (Cat. No. CR-M26901) (top panel).The precipitated active Ga i was immunoblotted with an anti Gαi rabbit polyclonal  antibody (Cat. No. CR-M21006). The bottom panel shows the Western blot with anti tubulin of the cell lysates.