基于液滴的微流体技术是一种新兴的强大工具，适用于dPCR或单细胞分析等生物应用。作为微反应器的液滴，可以在微流控芯片中产生，具有非常高的吞吐量，可在较短的时间内进行大量的平行实验。
氟化油通常用作这类应用的连续相，因为其能够溶解和输送氧气，并且不溶解大多数化学和生化化学物。
FluoSurf 是一种生物相容性氟化表面活性剂，专为生产单分散油包水液滴而设计和优化。FluoSurf 可确保液滴的长期稳定性（即使在极端条件下也是如此）。
优势
● 稳定性
即使在dPCR或长期细胞培养实验等具有挑战性的条件下，也能实现单分散液滴群体和低液滴融合。
● 生物相容性
经细胞检测验证的生物相容性，适用于生物实体。
● 批量生产
我们的大批量生产能力使我们能够满足您的所有需求
● 较高纯度
在制造加工过程中去除杂质
● 泄露控制：液滴内的分散相物质较少量的渗透到液滴外部
● 批次间重现性
每个批次都遵循严格的质量控制分析，通过验证结构和微流体性能来确保一致性。
FluoSurf 在高温处理（例如PCR的加热循环）后仍保证液滴稳定性和群体单分散性。
应用领域
● 数字液滴PCR
基于对液滴内大量分隔PCR反应的统计分析的超灵敏核酸定量方法
● 单细胞分析
在受控环境下处理和分析单分散液滴群体中的分离细胞
● 筛选
在微滴内以高通量对细胞、基因或生物大分子进行受控操作、分析和分选。
● 合成生物学
使用微液滴作为细胞大小的隔间来模拟代谢反应。
● 控制化学和生物反应
微流体工具专门设计用于增强对封装在液滴内的反应的控制。
规格参数
产品名称 | FluoSurf -C/-O 表面活性剂 |
溶剂 | 氟油7500或40或135或200 |
分子式 | PFPE-b-PPO-PEO-PPO-b-PFPE |
分子量 | 7kDa<Mw<13kDa |
电荷属性 | 电中性 |
表面张力(4wt% in HFE7500) | 4 mN/m |
临界胶束浓度CMC(in HFE7500) | 0.2 w/w% |
毒性 | 未分类为危险品，提供MSDS文件。 |
生物兼容性 | 已通过浮游生物、酵母、大肠杆菌和哺乳动物细胞进行测试。 |
微流体芯片兼容性 | 建议对微流控芯片通道表面进行亲氟疏水表面处理 |
储存条件 | 表面活性剂以琥珀色玻璃瓶装运，以防止紫外线降解。 室温下保存1-4年。 |
案例1: 液滴产生和热循环对尺寸分布的影响
实验条件：氟油流速为5μL/min，水相流速为1.66μL/min。
4w/w%表面活性剂HFE7500
热循环条件：30个循环
95℃驻留30s
55℃驻留1min
72℃驻留5min
40℃驻留1min
热循环前
热循环后
FluoSurf在热循环期间允许液滴具有良好的稳定性
使用FluoSurf表面活性剂，热循环对液滴尺寸分布的影响十分有限。
案例2：保留性能
荧光素100μM的全/空液滴平均强度的演变
FluoSurf限制液滴之间的交换（4天后少于10%的荧光素交换）
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