α突触核蛋白（Alpha-Synuclein）, Tag Free

Human Alpha-Synuclein Pre-formed Fibrils Protein, Tag Free

分子别名（Synonym）

SNCA,NACP,PARK1,alpha-Synuclein

表达区间及表达系统（Source）

Human Alpha-Synuclein Pre-formed Fibrils, Tag Free (ALN-H5115) is expressed from E. coli cells. It contains AA Met 1 - Ala 140 (Accession # P37840-1).

Predicted N-terminus: Met 1

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 14.5 kDa.

应用说明（Application）

THT, cell assay, drug screening and other in vitro experiment.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product should be stored at -70°C or room temperature for short storage. Do not store fibrils on ice or at 4°C;

The unsonicated fibril is validated to be stable after storage at -70°C for 1 year under sterile conditions;

The sonicated fibril should be stored at -70°C for not more than 8 weeks.

背景介绍

Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD). Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.