ARAP1抗体;ARAP1 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P11475(ARAP1 Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

The ARAP1 (ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1) antibody is a crucial tool for studying the function and expression of the ARAP1 protein, a multidomain signaling molecule involved in regulating cellular processes such as cytoskeletal reorganization, membrane trafficking, and cell adhesion. ARAP1 acts as a dual GTPase-activating protein (GAP) for both Arf and Rho family GTPases, modulating their activity to control intracellular signaling pathways. It plays roles in insulin signaling, endocytic trafficking, and cell migration, with implications in metabolic disorders, cancer, and immune regulation.

ARAP1 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect ARAP1 expression levels and localization in tissues or cultured cells. Researchers employ these antibodies to investigate ARAP1's involvement in diseases such as diabetes, where ARAP1 polymorphisms are linked to insulin resistance, or in cancer metastasis due to its regulatory effects on cell motility. Commercial ARAP1 antibodies are typically raised against specific epitopes, often within its N-terminal region or functional domains, and validated for species reactivity (e.g., human, mouse). Proper validation via knockout controls or siRNA knockdown is essential to ensure specificity, given ARAP1's structural complexity and homology with other GAP proteins.