RUSC1抗体;RUSC1 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P10160(RUSC1 Antibody) at dilution 1/90. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P10160(RUSC1 Antibody) at dilution 1/90. (Original magnification: ×200)    

  
  

The RUSC1 (RUN and SH3 domain-containing protein 1) antibody is a tool used to study the RUSC1 protein, which plays roles in intracellular vesicular trafficking, neuronal development, and signaling pathways. RUSC1 contains an N-terminal RUN domain, implicated in membrane trafficking, and a C-terminal SH3 domain involved in protein-protein interactions. It interacts with components of the autophagy-lysosome pathway, such as WDR44/Atg21. and regulates endosomal sorting and cargo degradation. Dysregulation of RUSC1 has been linked to neurological disorders and cancers, making it a target for mechanistic studies.

RUSC1 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides derived from conserved regions of the human RUSC1 sequence. These antibodies are validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation to detect endogenous RUSC1 expression in cell lines or tissues. Specificity is confirmed via knockout (KO) controls or siRNA knockdown. Commercial RUSC1 antibodies often target epitopes within the central or C-terminal regions. Researchers use these antibodies to explore RUSC1's role in Golgi-lysosome communication, neuronal differentiation, and diseases like glioblastoma or Alzheimer's. Variants like the phosphorylated forms may require specialized antibodies. Proper validation remains critical due to potential cross-reactivity with paralogs like RUSC2.