ECSIT抗体;ECSIT Antibody—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Jurkat and HepG2 cell lysates, Primary antibody: P10159(ECSIT Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P10159(ECSIT Antibody) at dilution 1/70. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P10159(ECSIT Antibody) at dilution 1/70. (Original magnification: ×200)    

  
  

ECSIT (Evolutionarily Conserved Signaling Intermediate in Toll pathways) is a multifunctional adaptor protein critical in regulating innate immune responses, mitochondrial function, and cellular signaling pathways. Initially identified as a mediator in Toll-like receptor (TLR) signaling, ECSIT interacts with components like TRAF6 and TAK1 to activate NF-κB and MAPK pathways, thereby modulating inflammatory cytokine production and immune cell activation. Beyond immunity, ECSIT plays a vital role in mitochondrial respiration by facilitating the assembly of Complex I in the electron transport chain, impacting cellular energy metabolism and reactive oxygen species (ROS) regulation. It also participates in BMP (bone morphogenetic protein) signaling, influencing embryonic development and tissue homeostasis.

ECSIT antibodies are essential research tools for detecting and analyzing ECSIT expression, localization, and interactions in various biological contexts. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study ECSIT’s involvement in immune disorders, mitochondrial diseases, cancer, and neurodegenerative conditions. For example, ECSIT dysregulation has been linked to chronic inflammation, tumor progression, and defective mitochondrial function. These antibodies help elucidate molecular mechanisms, such as how ECSIT bridges TLR signaling to mitochondrial ROS production or its role in mitophagy. Species-specific ECSIT antibodies (e.g., human, mouse) enable cross-species comparative studies, aiding in translational research. Overall, ECSIT antibodies are pivotal in exploring the protein’s dual roles in immunity and metabolism, offering insights into therapeutic targets for related diseases.