GLO1抗体;GLO1 Antibody—艾普蒂

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     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human prostate tissue, Raji cells, Primary antibody: P08371(GLO1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P08371(GLO1 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P08371(GLO1 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The glyoxalase 1 (GLO1) antibody is a crucial tool in studying the glyoxalase system, a key enzymatic pathway involved in detoxifying methylglyoxal (MG), a highly reactive byproduct of glycolysis. GLO1. a zinc-dependent metalloenzyme, works with glyoxalase 2 (GLO2) to convert MG into D-lactate using glutathione as a cofactor. Elevated MG levels are linked to oxidative stress, protein glycation, and cellular damage, contributing to pathologies like diabetes, neurodegenerative diseases, cancer, and aging. GLO1's role in mitigating MG toxicity makes it a biomarker and therapeutic target in these conditions.

GLO1 antibodies are widely used in research to detect and quantify GLO1 expression in tissues and cells via techniques such as Western blotting, immunohistochemistry, and ELISA. They help investigate GLO1's regulation under stress, metabolic changes, or disease states. Commercial GLO1 antibodies are typically raised against recombinant human or murine GLO1 proteins, available as monoclonal or polyclonal forms. Recent studies explore GLO1's dual role: while its upregulation in some cancers promotes cell survival, its downregulation in neurodegenerative contexts exacerbates oxidative injury. Challenges include ensuring antibody specificity due to structural similarities among glutathione-dependent enzymes. Ongoing research focuses on developing inhibitors or activators of GLO1 activity, with antibodies playing a pivotal role in validating target engagement and mechanistic studies.