DNAJC15抗体;DNAJC15 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P08367(DNAJC15 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The DNAJC15 antibody is a research tool designed to detect and study DNAJC15. a member of the DnaJ/Hsp40 protein family. DNAJC15. also known as Tid1 or mitochondrial DnaJ protein, functions as a co-chaperone for Hsp70 proteins, aiding in protein folding, quality control, and mitochondrial homeostasis. It is localized primarily in mitochondria and plays roles in apoptosis, cellular stress responses, and energy metabolism. Dysregulation of DNAJC15 has been linked to cancer, neurodegenerative diseases, and metabolic disorders, making it a target for mechanistic and therapeutic studies.

Antibodies against DNAJC15 are generated using immunogenic peptide regions of the protein, often in hosts like rabbits or mice. These antibodies enable detection of endogenous DNAJC15 in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Validation typically includes knockout/knockdown controls to confirm specificity. Commercial antibodies vary in clonality (monoclonal/polyclonal), epitope targets, and applications, requiring optimization for experimental conditions like fixation or sample preparation.

Research utilizing DNAJC15 antibodies has elucidated its interaction with mitochondrial complexes, involvement in stress-induced apoptosis via the Bcl-2 pathway, and potential as a biomarker in diseases like prostate cancer. However, discrepancies in reported molecular weights (∼15-25 kDa) across studies highlight the importance of antibody validation and consideration of post-translational modifications.