UPK3B抗体;UPK3B Antibody—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Flow cytometric analysis of THP-1 cells using UPK3B mouse mAb (green) and negative control (red).    

  
  

The UPK3B (Uroplakin 3B) antibody is a tool used to detect the UPK3B protein, a member of the uroplakin family critical for maintaining the permeability barrier and mechanical stability of the urothelium, the epithelial lining of the urinary tract. Uroplakins, including UPK1A, UPK1B, UPK2. UPK3A, and UPK3B, form heterodimeric complexes that assemble into membrane plaques, contributing to the unique apical surface of umbrella cells. These structures prevent urine leakage and pathogen adhesion. UPK3B, specifically, pairs with UPK2 to form a key component of these plaques.

UPK3B antibodies are primarily utilized in research and diagnostics to study urothelial biology and pathologies. In normal tissues, UPK3B expression is restricted to the urothelium, making it a specific marker for identifying urothelial cells in histological samples. Its dysregulation is implicated in bladder cancers, with altered expression patterns observed in urothelial carcinomas. Researchers employ UPK3B antibodies in techniques like immunohistochemistry, Western blotting, and immunofluorescence to assess protein localization, expression levels, and potential biomarkers for cancer progression.

Additionally, UPK3B antibodies aid in studying congenital bladder disorders and urinary tract infections, where uroplakin integrity is compromised. Their specificity is validated using knockout models or siRNA-mediated silencing to ensure minimal cross-reactivity. Overall, UPK3B antibodies serve as vital reagents for exploring urothelial function, disease mechanisms, and therapeutic targets.