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  • NDC80抗体;NDC80 Antibody—艾普蒂
  • NDC80抗体;NDC80 Antibody—艾普蒂
  • NDC80抗体;NDC80 Antibody—艾普蒂

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NDC80抗体;NDC80 Antibody—艾普蒂

Mouse Monoclonal NDC80 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-05-25

上海切尔齐生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：nuodina88@163.com

产品详情:

中文名称：
NDC80抗体
英文名称：
Mouse Monoclonal NDC80 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 1448 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
NDC80

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesHEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
Entrez GeneID10403
clone4B12H3
WB Predicted band size74kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human NDC80 (AA: 443-642) expressed in Mammalian.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using NDC80 mouse mAb against Jurkat (1) cell lysate.    


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       Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of HepG2 cells using NDC80 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of Jurkat cells using NDC80 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of K562 cells using NDC80 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using NDC80 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using NDC80 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using NDC80 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using NDC80 mouse mAb with DAB staining.    


           

参考文献

以下是关于NDC80抗体的3-4篇参考文献示例(文献信息为模拟，仅供参考)：

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1. **文献名称**：*"The Ndc80 complex bridges microtubule-kinetochore interactions in mitosis"*

**作者**：Cheeseman, I.M. et al.

**摘要**：本研究利用NDC80抗体进行免疫沉淀和功能分析，阐明了NDC80复合体在染色体分离中连接微管与动粒的关键作用，并揭示其缺失导致染色体排列异常。

2. **文献名称**：*"Structure of the Ndc80 kinetochore complex and its role in microtubule binding"*

**作者**：Ciferri, C. et al.

**摘要**：通过冷冻电镜和抗体标记技术，解析了NDC80复合体的三维结构，证明其通过卷曲螺旋结构域与微管结合，为有丝分裂中动粒组装提供结构基础。

3. **文献名称**：*"NDC80 phosphorylation regulates kinetochore-microtubule attachments and mitotic progression"*

**作者**：DeLuca, J.G. et al.

**摘要**：利用磷酸化特异性NDC80抗体，发现Aurora B激酶介导的NDC80磷酸化调控动粒-微管附着的稳定性，确保染色体正确分离。

4. **文献名称**：*"NDC80 overexpression as a prognostic biomarker in breast cancer"*

**作者**：Wang, Y. et al.

**摘要**：通过免疫组化(NDC80抗体)分析乳腺癌组织，发现NDC80高表达与患者生存率低相关，提示其作为潜在肿瘤标志物的价值。

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注：以上文献信息为示例，实际引用时请核实真实文献的准确性。建议通过PubMed或Google Scholar搜索关键词“NDC80 antibody”或“NDC80 function”获取最新研究。

       

背景信息

The NDC80 antibody is a crucial tool for studying the NDC80 complex, a conserved heterotetrameric protein assembly (comprising NDC80/HEC1. NUF2. SPC24. and SPC25) essential for proper chromosome segregation during mitosis. This complex forms part of the KMN network (KNL1. MIS12. NDC80) at kinetochores, mediating stable microtubule attachment and ensuring accurate chromosome alignment on the mitotic spindle. NDC80 antibodies are widely used to investigate mitotic mechanisms, checkpoint signaling, and errors linked to chromosomal instability—a hallmark of cancer and developmental disorders.

Researchers employ these antibodies in techniques like immunofluorescence, Western blotting, and immunoprecipitation to visualize NDC80 localization, quantify expression levels, or study protein-protein interactions. Specific epitopes targeted by commercial antibodies (e.g., recognizing human NDC80/HEC1’s N-terminal region) help dissect structural-functional relationships. Studies using NDC80 antibodies have revealed its role in correcting microtubule-kinetochore misattachments and maintaining tension across sister chromatids. Dysregulation of NDC80 is implicated in tumorigenesis, making it a potential biomarker or therapeutic target. However, antibody validation remains critical, as off-target binding can complicate interpretations in diverse experimental models.

       
NDC80抗体;NDC80;NDC80 Antibody;

公司简介

生物技术有限公司

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,植物生物技术,细胞培养,蛋白组学

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