USP7抗体;USP7 Antibody—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using USP7 mouse mAb against Hela (1), A431 (2), MCF-7 (3),Jurkat (4),K562 (5),HepG2 (6),A549 (7),HCT116 (8),HT-29 (9),SW480 (10),C6 (11),COS-7 (12),and NIH/3T3 (13) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using mouse USP7 mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using USP7 mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of HepG2 cells using USP7 mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of Jurlat cells using USP7 mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of K562 cells using USP7 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using USP7 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using USP7 mouse mAb with DAB staining.    

  
  

USP7 (Ubiquitin-Specific Protease 7), also known as HAUSP, is a deubiquitinating enzyme critical for regulating protein stability and function by removing ubiquitin moieties from target proteins. It plays a central role in diverse cellular processes, including DNA repair, epigenetic regulation, immune response, and apoptosis. USP7 is particularly notable for its interaction with tumor suppressors (e.g., p53. PTEN) and oncoproteins (e.g., MDM2. viral proteins), making it a key player in cancer biology, neurodegenerative diseases, and viral pathogenesis.

USP7-specific antibodies are essential tools for studying its expression, localization, and molecular mechanisms. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate USP7’s role in substrate recognition, post-translational modifications, and pathway modulation. High-quality USP7 antibodies must exhibit specificity to avoid cross-reactivity with other ubiquitin-specific proteases (e.g., USP5. USP13) due to structural similarities within the USP family. Validation via knockout cell lines or siRNA-mediated depletion is critical to confirm target specificity.

Research on USP7 antibodies has accelerated drug discovery efforts, as USP7 inhibitors are being explored for cancer therapy and antiviral treatments. Reliable antibodies help dissect USP7’s dual roles in promoting or suppressing disease, depending on cellular context, underscoring its therapeutic potential and complexity as a biological target.