CD317抗体;CD317 Antibody—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using CD317 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of THP-1 cells using CD317 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using CD317 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using CD317 mouse mAb with DAB staining.    

  
  

CD317. also known as Tetherin or BST-2 (bone marrow stromal antigen 2), is a transmembrane protein encoded by the *BST2* gene. It plays a critical role in innate immunity by restricting the release of enveloped viruses, including HIV-1. Ebola, and SARS-CoV-2. through its unique ability to physically "tether" budding virions to the host cell membrane. Structurally, CD317 features an N-terminal cytoplasmic domain, a transmembrane region, and a C-terminal glycosylphosphatidylinositol (GPI) anchor, enabling it to form parallel dimers that crosslink viral and cellular membranes.

Antibodies targeting CD317 are essential tools for studying its antiviral mechanisms, expression patterns, and interactions with viral proteins (e.g., HIV-1 Vpu, which counteracts CD317 by promoting its degradation). These antibodies are widely used in techniques like flow cytometry, Western blotting, and immunohistochemistry to investigate CD317's role in viral restriction, immune signaling, and cancer biology. Notably, CD317 is overexpressed in certain cancers, such as multiple myeloma, and its antibodies aid in exploring its dual role as both a tumor suppressor and promoter. Research also focuses on CD317's involvement in cell adhesion, NF-κB signaling, and its potential as a therapeutic target. However, its complex regulation and context-dependent functions necessitate careful interpretation of antibody-based assays. Overall, CD317 antibodies remain pivotal in deciphering the interplay between host defense, viral evasion, and disease progression.