IL1A抗体;IL1A Antibody—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using IL1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of HL-60 cells using IL1A mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of Jurkat cells using IL1A mouse mAb (green) and negative control (red).    

  
  

Interleukin-1 alpha (IL-1α) is a pro-inflammatory cytokine belonging to the IL-1 family, encoded by the IL1A gene. It is produced as a precursor protein (pro-IL-1α) that is cleaved into its active form, primarily by calpain-like proteases. IL-1α binds to the IL-1 receptor (IL-1R), triggering downstream signaling pathways (e.g., NF-κB, MAPK) that drive inflammatory and immune responses. Unlike IL-1β, IL-1α is constitutively expressed in epithelial and stromal cells and can act as both a secreted cytokine and an intracellular "alarmin" released during cell damage to signal tissue stress. Its dual role in homeostasis and inflammation makes it a key mediator in infections, autoimmune diseases, and cancer.

IL-1α-specific antibodies are critical tools for studying its expression, localization, and function. These antibodies are widely used in techniques like ELISA, Western blotting, immunohistochemistry, and flow cytometry to quantify IL-1α levels in biological samples or visualize its distribution in tissues. Neutralizing IL-1α antibodies have therapeutic potential, as excessive IL-1α signaling is implicated in rheumatoid arthritis, atherosclerosis, and inflammatory skin disorders. Research-grade antibodies also help distinguish IL-1α from IL-1β in mechanistic studies, given their overlapping pathways but distinct roles. Recent studies explore IL-1α's contribution to the tumor microenvironment, highlighting the importance of reliable antibodies in both basic research and clinical applications.