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网站主页 Mouse Monoclonal Vimentin Antibody Mouse Monoclonal Vimentin Antibody
  • Vimentin抗体;Vimentin Antibody—艾普蒂
  • Vimentin抗体;Vimentin Antibody—艾普蒂
  • Vimentin抗体;Vimentin Antibody—艾普蒂

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Vimentin抗体;Vimentin Antibody—艾普蒂

Mouse Monoclonal Vimentin Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-05-25

上海切尔齐生物科技有限公司

非会员
联系人：周***
电话：027***
手机：180***
邮箱：nuo***

产品详情:

中文名称：
Mouse Monoclonal Vimentin Antibody
英文名称：
Mouse Monoclonal Vimentin Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 1300 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
Vimentin

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200-1/1000 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM1/200-1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesFLJ36605; VIM
Entrez GeneID7431
clone3A1F2
WB Predicted band size53.7kDa
Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenPurified recombinant fragment of human VIM (AA: 2-466) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)    


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       Western blot analysis using VIM mouse mAb against Jurkat (1), K562 (2), SK-N-SH (3), SH-SY5Y (4), Hela (5), NIH/3T3 (6), C6 (7), and RAW264.7 (8) cell lysate.    


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       Flow cytometric analysis of Hela cells using VIM mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using VIM mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using VIM mouse mAb with DAB staining.    


           

参考文献

以下是3篇与Vimentin抗体相关的代表性文献(示例基于真实研究，具体引用需核实)：

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1. **标题**: *"Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer"*

**作者**: Battaglia R.A. et al.

**摘要**: 研究利用Vimentin抗体通过免疫印迹和免疫荧光，证明Vimentin通过调控Axl表达促进乳腺癌细胞的上皮-间质转化(EMT)和迁移，揭示其在肿瘤转移中的机制。

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2. **标题**: *"Vimentin as a potential marker for circulating tumor cells in epithelial cancers"*

**作者**: Satelli A. et al.

**摘要**: 该文献通过免疫细胞化学分析，验证Vimentin抗体在检测上皮源性肿瘤循环细胞(CTCs)中的应用，证明其作为间充质表型标志物在癌症预后评估中的价值。

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3. **标题**: *"Antibody-based profiling of intermediate filaments in human cell lines"*

**作者**: Löwe J. et al.

**摘要**: 系统评估多种中间丝蛋白抗体(包括Vimentin)在人类细胞系中的特异性，为细胞骨架研究提供标准化实验方案，强调抗体选择对结果可靠性的影响。

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**提示**：建议通过PubMed或Google Scholar搜索最新文献，使用关键词“Vimentin antibody application”或“Vimentin marker”筛选高被引论文，并优先选择《Journal of Cell Science》《Cancer Research》等期刊的研究。

       

背景信息

Vimentin antibodies are essential tools in cellular and molecular biology research, primarily used to detect vimentin, a type III intermediate filament protein. Vimentin is a key structural component of the cytoskeleton in mesenchymal cells, contributing to cell integrity, migration, and intracellular signaling. It is expressed in fibroblasts, endothelial cells, immune cells, and certain epithelial cells during processes like epithelial-mesenchymal transition (EMT), a critical mechanism in development, wound healing, and cancer metastasis.

These antibodies enable researchers to study vimentin's role in diseases, particularly cancers (e.g., sarcomas, carcinomas) and fibrotic disorders, where its overexpression correlates with aggressive phenotypes. In diagnostics, vimentin antibodies assist in identifying cell origins in tumor samples, distinguishing mesenchymal tumors from epithelial ones.

Available as monoclonal or polyclonal forms, vimentin antibodies are validated for techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting. Species reactivity typically includes human, mouse, and rat. Researchers must select antibodies based on target applications, ensuring compatibility with fixation methods and sample types. Proper controls are crucial, as cross-reactivity with other intermediate filaments (e.g., desmin) may occur.

Overall, vimentin antibodies are pivotal in unraveling cytoskeletal dynamics, disease mechanisms, and cellular plasticity, bridging basic research and clinical diagnostics.

       
Vimentin抗体;Vimentin;Vimentin Antibody;

公司简介

生物技术有限公司

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,植物生物技术,细胞培养,蛋白组学

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