AMBRA1抗体;AMBRA1 Antibody—艾普蒂

+ +

•  

   

     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

•  

   

     Flow cytometric analysis of Hela cells using AMBRA1 mouse mAb (green) and negative control (red).    

  
  

The AMBRA1 (Autophagy and Beclin 1 Regulator 1) antibody is a key tool for studying the molecular mechanisms of autophagy, a cellular degradation process critical for maintaining homeostasis. AMBRA1 is a pro-autophagic protein that regulates the formation of autophagosomes by interacting with Beclin 1 and the PI3K complex, promoting autophagy initiation. It also acts as a substrate receptor for the E3 ubiquitin ligase CUL4A-DDB1. targeting proteins for degradation via the ubiquitin-proteasome system. Dysregulation of AMBRA1 is implicated in neurodevelopmental disorders, cancer, and neurodegenerative diseases, as it influences cell proliferation, apoptosis, and neuronal development.

The AMBRA1 antibody is widely used in research to detect and quantify AMBRA1 expression in tissues or cell lines using techniques like Western blotting, immunoprecipitation, or immunofluorescence. It helps elucidate AMBRA1's role in balancing autophagy and apoptosis, its interaction with signaling pathways (e.g., mTOR), and its tumor-suppressive functions. Recent studies highlight its involvement in mitophagy and ciliogenesis, expanding its relevance in cellular quality control. Reliable AMBRA1 antibodies are essential for exploring its dual roles in health and disease, offering insights into therapeutic strategies targeting autophagy-related pathways.