PSMF1抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: HEPG2 and Hela cell, Primary antibody: P09100(PSMF1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P09100(PSMF1 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The PSMF1 (Proteasome Inhibitor 31 kDa Subunit Family Member 1) antibody is a tool used to study the PSMF1 protein, a regulator of proteasome activity. PSMF1. also known as PI31. binds to the 26S proteasome and modulates its function, influencing protein degradation pathways critical for cellular homeostasis. This protein is implicated in controlling proteasome assembly, substrate processing, and crosstalk between proteasomes and autophagy. Dysregulation of PSMF1 is linked to neurodegenerative diseases, cancer, and immune disorders, making it a focus in pathophysiological research.

PSMF1 antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides as immunogens. They are validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous PSMF1 in human, mouse, or rat tissues. Commercial antibodies often include epitope specificity data and are used to explore PSMF1’s expression patterns, interactions (e.g., with proteasome subunits or autophagy-related proteins), and roles in stress responses or apoptosis. Researchers also employ these antibodies to investigate PSMF1’s dual role as both a proteasome inhibitor and activator under varying cellular conditions. Quality validation typically involves knockout controls or siRNA-mediated silencing to confirm signal specificity. Studies using PSMF1 antibodies have advanced understanding of its involvement in diseases like Alzheimer’s, multiple myeloma, and inflammation.