RSPH1抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human testis tissue, Primary antibody: P09099(RSPH1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P09099(RSPH1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The RSPH1 (radial spoke head protein 1) antibody is a tool used to study the RSPH1 protein, a component of the radial spoke head in motile cilia and flagella. Radial spokes are critical for regulating ciliary movement by transmitting signals between the central pair of microtubules and dynein arms. RSPH1. encoded by the *RSPH1* gene (located on human chromosome 21q22.3), is part of a multi-protein complex that stabilizes ciliary structure and coordinates beating patterns. Mutations in *RSPH1* are linked to primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, leading to chronic respiratory infections, sinusitis, and infertility.

Antibodies targeting RSPH1 are primarily used in research to investigate ciliary ultrastructure, protein interactions, and disease mechanisms. They enable techniques like immunofluorescence, Western blotting, or immunohistochemistry to visualize RSPH1 localization in ciliated tissues (e.g., respiratory epithelium, sperm flagella) or cell models. Commercial RSPH1 antibodies are often raised in rabbits or mice using peptide antigens derived from conserved regions of the protein. Validation typically includes knockout cell lines or PCD patient samples with confirmed *RSPH1* mutations. Recent studies also explore RSPH1's potential role beyond PCD, such as in developmental processes or cancer, where ciliary dysfunction may contribute to pathogenesis.