UQCRH抗体—艾普蒂

+ +

•  

   

     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Mouse heart tissue lysate, Primary antibody: P09045(UQCRH Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 30 seconds    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P09045(UQCRH Antibody) at dilution 1/90. (Original magnification: ×200)    

  
  

The UQCRH (Ubiquinol-cytochrome c reductase hinge protein) antibody is a tool used to detect and study the UQCRH protein, a critical subunit of mitochondrial Complex III (cytochrome bc1 complex) in the electron transport chain (ETC). Located in the inner mitochondrial membrane, Complex III facilitates electron transfer from ubiquinol to cytochrome c while contributing to the proton gradient essential for ATP synthesis. UQCRH, encoded by the nuclear UQCRH gene, acts as a structural and functional stabilizer of Complex III, ensuring its proper assembly and interaction with other ETC components.

Research involving UQCRH antibodies focuses on understanding mitochondrial dysfunction linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. For example, altered UQCRH expression has been observed in certain cancers, suggesting its role in tumor metabolism and apoptosis regulation. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression levels, localization, and interactions in cellular and tissue samples.

UQCRH antibodies are typically raised in host species (e.g., rabbits or mice) using immunogenic peptide sequences specific to conserved regions of the protein. Validation includes testing for specificity via knockdown/knockout controls. Their application extends to both basic research and clinical studies, aiding in the exploration of mitochondrial biology and therapeutic targeting of ETC-related pathologies.