NDUFB9抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Hela cell and human fetal liver tissue, A549 and Jurkat cell, Primary antibody: P09037(NDUFB9 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

The NDUFB9 antibody is a crucial tool for studying the mitochondrial complex I (NADH:ubiquinone oxidoreductase), a key enzyme in the electron transport chain responsible for cellular ATP production. NDUFB9 (NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex 9) is a nuclear-encoded subunit of complex I, located in the mitochondrial inner membrane. This hydrophobic protein plays a structural role in complex I assembly and stability, though its exact catalytic function remains under investigation.

Antibodies targeting NDUFB9 are widely used in research to investigate mitochondrial disorders, metabolic diseases, and cancer metabolism. They enable detection of NDUFB9 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry, helping assess complex I integrity in conditions like Leigh syndrome, Parkinson’s disease, or chemotherapy-induced mitochondrial dysfunction. Commercial NDUFB9 antibodies are typically raised against specific peptide epitopes and validated for specificity across human and model organisms. Recent studies also utilize these antibodies to explore tissue-specific complex I variations and screen for mitochondrial toxicity in drug development. Proper validation using knockout controls is essential, as complex I subunits often share structural homology, requiring careful interpretation of results.