NDUFB10抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human fetal muscle tissue, Raji and Jurkat cell, Primary antibody: P09033(NDUFB10 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P09033(NDUFB10 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The NDUFB10 antibody is a crucial tool for studying the NDUFB10 protein, a subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) within the electron transport chain (ETC). Complex I, located in the mitochondrial inner membrane, plays a vital role in oxidative phosphorylation by transferring electrons from NADH to ubiquinone, coupled with proton translocation to drive ATP synthesis. NDUFB10. encoded by the nuclear gene NDUFB10. is an accessory subunit critical for Complex I assembly, stability, and enzymatic activity. Dysregulation of NDUFB10 has been linked to mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s), and cancer, where altered ETC function impacts cellular metabolism.

NDUFB10 antibodies are typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies enable detection and quantification of NDUFB10 in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. They are essential for investigating mitochondrial dysfunction mechanisms, assessing protein expression in disease models, and validating gene-editing outcomes (e.g., CRISPR/Cas9 knockouts). Specificity is validated via controls like knockout cell lines or siRNA knockdown. Researchers also use these antibodies to explore metabolic reprogramming in cancer or evaluate therapeutic responses targeting mitochondrial pathways. High-quality NDUFB10 antibodies ensure reliable data, advancing studies on cellular bioenergetics and disease pathogenesis.