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  • ZNHIT1抗体—艾普蒂
  • ZNHIT1抗体—艾普蒂
  • ZNHIT1抗体—艾普蒂

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ZNHIT1抗体—艾普蒂

Rabbit Polyclonal ZNHIT1 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期:2025-05-19

上海切尔齐生物科技有限公司

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产品详情:

中文名称:
ZNHIT1抗体
英文名称:
Rabbit Polyclonal ZNHIT1 Antibody
品牌:
艾普蒂
产地:
武汉
保存条件:
Stored at -20°C for 4527 year. Avoid repeated freeze / thaw cycles.
产品类别:
抗体
重组:
应用:
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性:
Human,Mouse,Rat
宿主:
Rabbit
偶联物:
靶点:
ZNHIT1

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/30-1/150 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/5000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesCG1I; ZNFN4A1
WB Predicted band size18 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFull length fusion protein
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: 293T cell lysate , Primary antibody: P09026(ZNHIT1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    


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       The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P09026(ZNHIT1 Antibody) at dilution 1/40. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P09026(ZNHIT1 Antibody) at dilution 1/40. (Original magnification: ×200)    


           

参考文献

以下是几篇可能涉及ZNHIT1抗体的参考文献示例(注:部分文献信息为模拟,建议通过学术数据库核实):

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1. **"ZNHIT1 regulates chromatin remodeling in DNA damage response"**

*Authors: Chen L, Wang X, et al.*

**摘要**:本研究通过免疫沉淀(IP)和Western blot技术,利用ZNHIT1特异性抗体证实了其在DNA损伤修复中与BRCA1复合物的相互作用,揭示了ZNHIT1通过调控染色质重塑影响同源重组修复的分子机制。

2. **"ZNHIT1 deficiency promotes tumorigenesis in hepatocellular carcinoma"**

*Authors: Tanaka K, Suzuki R, et al.*

**摘要**:文章通过免疫组化(IHC)分析肝癌组织中ZNHIT1蛋白表达,发现其表达水平与患者预后相关。使用shRNA敲低ZNHIT1后,Western blot验证了抗体特异性,并证明其缺失通过激活Wnt/β-catenin通路促进肿瘤进展。

3. **"Structural insights into the ZNHIT1-H2A.Z interaction by cryo-EM"**

*Authors: Müller M, Schmidt A, et al.*

**摘要**:利用ZNHIT1抗体进行蛋白纯化,结合冷冻电镜解析了ZNHIT1与组蛋白变体H2A.Z的复合物结构,阐明了其介导染色质动态调控的结构基础。

4. **"ZNHIT1 as a novel biomarker for ovarian cancer chemoresistance"**

*Authors: Li Y, Zhang H, et al.*

**摘要**:通过流式细胞术和免疫荧光检测卵巢癌细胞中ZNHIT1的亚定位,发现其核质移位与顺铂耐药相关,抗体阻断实验进一步验证了其在化疗敏感性中的功能。

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**注意**:上述文献为示例性质,实际引用时请通过PubMed、Google Scholar等平台以“ZNHIT1 antibody”或“ZNHIT1 function”为关键词检索最新研究,并核对作者及期刊信息。

       

背景信息

The ZNHIT1 antibody is a research tool designed to detect Zinc Finger HIT-Type Containing 1 (ZNHIT1), a protein encoded by the ZNHIT1 gene. ZNHIT1. also known as p18 hamlet or HSPC324. is a conserved nuclear protein belonging to the HIT (histone triad) superfamily, characterized by a zinc-binding HIT domain. It plays roles in chromatin remodeling, transcriptional regulation, and DNA repair by interacting with histone chaperones and chromatin-modifying complexes. Specifically, ZNHIT1 is a critical component of the SHREC (Snf2 histone linker ejection chaperone) complex, which regulates histone H1 dynamics and heterochromatin formation. It also interacts with the p53 tumor suppressor pathway, influencing cell cycle control and apoptosis.

ZNHIT1 antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study protein expression, localization, and molecular interactions. Dysregulation of ZNHIT1 has been linked to cancers, neurodevelopmental disorders, and premature aging syndromes, making its antibody valuable for investigating disease mechanisms. Researchers validate these antibodies for specificity using knockout cell lines or siRNA-mediated knockdown. Commercial ZNHIT1 antibodies are typically raised in rabbits or mice against synthetic peptides corresponding to conserved regions of the human protein. Proper controls are essential to ensure accurate interpretation in experimental models.

       
ZNHIT1抗体;ZNHIT1;ZNHIT1 Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业,

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,蛋白组学,细胞生物学

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