WWP1抗体—艾普蒂

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 231 and hela cell, Primary antibody: P09005(WWP1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P09005(WWP1 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

**Background of WWP1 Antibody**

The WWP1 antibody is a crucial tool for studying the biological functions of WWP1 (WW Domain-Containing E3 Ubiquitin Protein Ligase 1), a member of the NEDD4-like family of HECT-type E3 ubiquitin ligases. WWP1 plays a pivotal role in regulating protein ubiquitination, a post-translational modification essential for protein degradation, localization, and signaling. Structurally, WWP1 contains a C2 domain (mediating membrane binding), multiple WW domains (for substrate recognition), and a catalytic HECT domain. It interacts with various substrates, including tumor suppressors (e.g., PTEN, SMADs) and oncogenic targets (e.g., EGFR), influencing processes like cell growth, apoptosis, and cancer progression. Dysregulation of WWP1 is implicated in cancers, neurodegenerative diseases, and metabolic disorders.

WWP1 antibodies are designed to detect endogenous WWP1 protein levels, localization, or post-translational modifications in experimental models. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). These antibodies are often validated using knockout/knockdown controls to ensure specificity. Some target specific regions (e.g., N-terminal or HECT domains) to study domain-specific interactions or activity. Researchers rely on WWP1 antibodies to explore its roles in disease mechanisms, therapeutic targeting, and ubiquitin-proteasome system dynamics, making them indispensable in both basic and translational studies.