USF1抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P08955(USF1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The USF1 (Upstream Stimulatory Factor 1) antibody is a crucial tool for studying the USF1 protein, a transcription factor belonging to the basic helix-loop-helix leucine zipper (bHLH-LZ) family. USF1 regulates gene expression by binding to E-box motifs (CACGTG) in promoter regions, often forming heterodimers with USF2. It plays roles in diverse cellular processes, including metabolism, cell cycle regulation, and DNA damage response. USF1 is implicated in diseases like diabetes, cardiovascular disorders, and cancer due to its influence on genes such as those encoding lipoproteins, insulin receptors, and tumor suppressors (e.g., p53).

USF1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to detect protein expression, localization, and DNA-binding activity. These antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Researchers rely on them to explore USF1's interactions with cofactors, its role in epigenetic regulation, and its dysregulation in pathological conditions. Validation via knockout controls or siRNA knockdown is essential to ensure specificity, as cross-reactivity with USF2 or other bHLH-LZ proteins may occur. USF1 antibodies remain vital for dissecting its complex regulatory networks and therapeutic potential.