MED6抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Hela and PC3 cell, 231 cell and human esophagus cancer tissue, Primary antibody: P08906(MED6 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P08906(MED6 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The MED6 antibody is a crucial tool for studying the Mediator complex, a multi-protein assembly essential for regulating RNA polymerase II (RNAP II)-mediated transcription in eukaryotes. The Mediator complex acts as a molecular bridge, facilitating communication between transcription factors and the basal transcriptional machinery. MED6. a core subunit within the Mediator’s middle module, plays a structural and functional role in stabilizing the complex and modulating its interactions with RNAP II. Research on MED6 has provided insights into transcriptional initiation, elongation, and epigenetic regulation.

Antibodies targeting MED6 are widely used in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate its expression, localization, and dynamics under various cellular conditions. Studies employing MED6 antibodies have revealed its involvement in developmental processes, stress responses, and diseases such as cancer, where Mediator subunits are often dysregulated. For example, altered MED6 expression has been linked to tumor progression and drug resistance.

MED6 antibodies also aid in exploring evolutionary conservation, as homologs exist in yeast (Med6) and other model organisms. These tools help dissect Mediator’s architecture and its role in gene-specific or genome-wide regulatory mechanisms. Challenges include ensuring antibody specificity due to the complex’s structural heterogeneity. Overall, MED6 antibodies remain pivotal in advancing our understanding of transcriptional regulation and its implications in health and disease.