STK33抗体—艾普蒂

The serine/threonine kinase 33 (STK33) is a calcium/calmodulin-regulated enzyme belonging to the CAMK (calcium/calmodulin-dependent protein kinase) family. It plays roles in cellular processes like cell cycle regulation, DNA damage response, and apoptosis. STK33 gained attention due to its potential oncogenic properties, particularly in cancers driven by KRAS mutations, where its kinase activity was initially proposed as critical for survival—though this remains debated. STK33 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect STK33 protein levels in tissues or cell lines. Researchers also employ STK33-specific antibodies for co-immunoprecipitation to identify interacting partners, aiding in mapping signaling pathways. Commercially available antibodies vary in host species (e.g., rabbit, mouse), clonality (monoclonal/polyclonal), and epitope specificity, necessitating validation for each application. Challenges include ensuring minimal cross-reactivity with homologous kinases. Recent studies suggest STK33 may influence spermatogenesis and metabolic regulation, expanding its research scope. Its role as a therapeutic target, particularly in oncology, drives ongoing investigations, with antibodies serving as critical reagents to validate STK33 inhibition strategies in preclinical models.