SNAP29抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P08789(SNAP29 Antibody) at dilution 1/45. (Original magnification: ×200)    

  
  

**Background of SNAP29 Antibody**

SNAP29 (Synaptosomal-Associated Protein 29 kDa) is a member of the SNARE (Soluble NSF Attachment Protein Receptor) family, which mediates membrane fusion events critical for intracellular trafficking, organelle biogenesis, and autophagy. Unlike its homologs SNAP23 and SNAP25. SNAP29 lacks a transmembrane domain and palmitoylation sites, enabling broad subcellular localization, including endosomes, lysosomes, and the Golgi apparatus. It functions as a non-neuronal SNARE, interacting with syntaxins and VAMP proteins to facilitate vesicle docking and fusion.

SNAP29 antibodies are essential tools for studying its role in cellular processes like autophagosome-lysosome fusion, epidermal barrier formation, and cytokine secretion. Dysregulation of SNAP29 is linked to pathologies such as CEDNIK syndrome (a neurocutaneous disorder), cancer progression, and neurodegenerative diseases. Antibodies targeting SNAP29 (e.g., monoclonal or polyclonal) are validated for applications including Western blotting, immunofluorescence, and co-immunoprecipitation to assess protein expression, localization, and interaction partners.

Specificity remains a key consideration due to homology with other SNAREs. Researchers often validate antibodies using knockout cell lines or siRNA knockdown. Additionally, post-translational modifications (e.g., phosphorylation) may affect antibody binding, necessitating careful experimental design. Overall, SNAP29 antibodies enable insights into membrane dynamics and disease mechanisms.