RRAGA抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hepg2 cells, hela cells, Primary antibody: P08725(RRAGA Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P08725(RRAGA Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

The RRAGA antibody is a research tool designed to detect and study the RRAGA protein, a member of the Ras-related GTP-binding protein family. RRAGA (Ras-related GTP-binding protein A), also known as RagA, is a critical component of the mTORC1 signaling pathway, which regulates cellular growth, metabolism, and autophagy in response to nutrient availability. As a GTPase, RRAGA cycles between GTP- and GDP-bound states, forming heterodimers with RagC/RagD to recruit mTORC1 to lysosomal surfaces, enabling its activation by amino acids and growth signals.

Antibodies targeting RRAGA are widely used in immunoblotting (Western blot), immunofluorescence, and immunoprecipitation to investigate its expression, subcellular localization, and interactions in various biological contexts. These reagents are essential for studying dysregulation of the mTOR pathway in diseases such as cancer, metabolic disorders, and neurodegenerative conditions. Researchers often validate RRAGA antibodies in knockout cell lines or tissues to confirm specificity, given the high homology among Rag family proteins. Commercial RRAGA antibodies are typically raised against unique epitopes in human or murine RRAGA, with cross-reactivity tested in multiple species. Their applications span basic mechanistic studies to drug development, particularly in therapies targeting mTORC1 hyperactivity.