RPLP1抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: K562 cells, hepg2 cells, Primary antibody: P08721(RPLP1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using P08721(RPLP1 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

The ribosomal protein large subunit P1 (RPLP1), also known as P1. is a component of the 60S ribosomal subunit and belongs to the acidic ribosomal phosphoprotein family. It plays a structural role in ribosome assembly and protein synthesis by stabilizing the ribosome's structure and interacting with translational machinery. RPLP1 is ubiquitously expressed and highly conserved across eukaryotes, reflecting its essential role in cellular function.

Antibodies targeting RPLP1 are widely used in research to study ribosome biogenesis, translation regulation, and cellular stress responses. They are valuable tools for techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect RPLP1 expression, localization, and interactions. Dysregulation of RPLP1 has been implicated in cancers, autoimmune diseases, and viral infections, making its antibody a critical reagent for investigating disease mechanisms.

Commercial RPLP1 antibodies are typically raised against specific epitopes, such as the C-terminal region, and are available as monoclonal or polyclonal forms. Validation includes testing on knockout cell lines or tissues to ensure specificity, given the high homology between RPLP1 and related family members (e.g., RPLP2/RPLP3). Challenges include cross-reactivity or batch variability, necessitating careful experimental optimization. Overall, RPLP1 antibodies contribute to understanding ribosome-related pathways and their roles in health and disease.