RNF214抗体—艾普蒂

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-4: 823 cells, PC3 cells, 231 cells, hepg2 cells, Primary antibody: P08715(RNF214 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P08715(RNF214 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The RNF214 antibody is a research tool designed to detect and study the Ring Finger Protein 214 (RNF214), a member of the E3 ubiquitin ligase family. RNF214 contains a characteristic RING (Really Interesting New Gene) domain, which facilitates its role in ubiquitination—a post-translational modification process critical for protein degradation, signaling, and cellular regulation. This protein is implicated in diverse biological processes, including cell cycle progression, DNA damage response, and immune regulation. Dysregulation of RNF214 has been linked to cancers, neurodegenerative disorders, and autoimmune diseases, though its precise mechanisms remain under investigation.

Antibodies targeting RNF214 are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for specificity through techniques such as Western blotting, immunofluorescence, and immunoprecipitation. Applications include detecting endogenous RNF214 expression in cell lines or tissues, studying its subcellular localization (e.g., cytoplasmic or nuclear distribution), and exploring interactions with substrates or binding partners in ubiquitination cascades. Researchers also utilize these antibodies to assess RNF214's role in disease pathways, such as tumorigenesis or inflammation, by analyzing expression patterns in pathological versus normal samples. Commercial RNF214 antibodies often come with validation data, including knockout/knockdown controls, to ensure reliability in experimental models.