RASSF3抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P08679(RASSF3 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P08679(RASSF3 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

The RASSF3 (RAS association domain family member 3) antibody is a crucial tool for studying the biological functions and pathological roles of the RASSF3 protein, a member of the RASSF tumor suppressor family. RASSF3. located on human chromosome 12q21.31. is involved in regulating cellular processes such as apoptosis, cell cycle progression, and microtubule stability. It interacts with key signaling pathways, including the Hippo and RAS/MAPK pathways, and modulates transcriptional activity through associations with proteins like MST2 and p53. Dysregulation of RASSF3 has been linked to cancer, where its downregulation or epigenetic silencing via promoter hypermethylation is observed in malignancies like hepatocellular carcinoma and colorectal cancer.

The RASSF3 antibody enables researchers to detect and quantify RASSF3 expression in various experimental settings, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Its specificity is critical for distinguishing RASSF3 from other RASSF family members (e.g., RASSF1. RASSF5), which share structural similarities but have distinct roles. Validated antibodies help elucidate tissue-specific expression patterns, subcellular localization (e.g., cytoplasmic vs. nuclear), and interactions in disease models. Commercially available RASSF3 antibodies are typically produced in hosts like rabbits or mice, with monoclonal or polyclonal options. Researchers must verify antibody performance using knockout controls or siRNA-based knockdown to ensure reliability, as inconsistent results across studies highlight potential cross-reactivity issues. Overall, the RASSF3 antibody is indispensable for advancing understanding of its tumor-suppressive mechanisms and therapeutic potential.