PLK4抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P08628(PLK4 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

PLK4 (Polo-like kinase 4) is a serine/threonine kinase belonging to the Polo-like kinase family, primarily involved in regulating centriole duplication and maintaining genomic stability during cell division. It plays a critical role in ensuring precise control of the centrosome cycle, which is essential for proper spindle formation and chromosome segregation. Dysregulation of PLK4 has been linked to various pathologies, including cancer, microcephaly, and ciliopathies, due to its impact on centrosome amplification or loss.

PLK4 antibodies are essential tools for studying its expression, localization, and function in both normal and diseased states. These antibodies enable researchers to detect PLK4 protein levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific monoclonal or polyclonal antibodies targeting distinct PLK4 epitopes help elucidate its phosphorylation status, interactions with substrates (e.g., STIL, CEP152), and regulatory mechanisms.

Recent studies highlight PLK4 as a potential therapeutic target, particularly in cancers with centrosome amplification. Inhibitors targeting PLK4 kinase activity are under investigation, and corresponding antibodies are critical for validating inhibitor efficacy and monitoring downstream effects. However, challenges remain in ensuring antibody specificity due to structural similarities among Polo-like kinases. Continued development of high-affinity, isoform-specific PLK4 antibodies will advance mechanistic studies and translational applications in oncology and developmental biology.