PGAM2抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: 293T cell, Human heart tissue, HepG2 cell, Jurkat and NIH/3T3 cell lysates, Primary antibody: P08615(PGAM2 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P08615(PGAM2 Antibody) at dilution 1/120. (Original magnification: ×200)    

  
  

The PGAM2 antibody is a crucial tool for studying the Phosphoglycerate Mutase 2 (PGAM2) enzyme, a key player in glycolysis. PGAM2. primarily expressed in skeletal muscle, catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate, facilitating ATP production. It belongs to the mutase family and exists as a homodimer or heterodimer with PGAM1. its ubiquitously expressed isoform. Dysregulation of PGAM2 is linked to metabolic disorders, including glycogen storage disease type X (GSD10), caused by PGAM2 mutations leading to exercise intolerance and myopathy.

PGAM2 antibodies are widely used in research to investigate enzyme expression, localization, and function in muscle metabolism, cancer (due to glycolysis’s role in tumorigenesis), and mitochondrial diseases. They enable detection via techniques like Western blot, immunohistochemistry, and immunofluorescence. Commercial PGAM2 antibodies are typically raised against specific epitopes, often validated for reactivity in human, mouse, or rat samples. Recent studies also explore PGAM2’s role in modulating oxidative stress and apoptosis, expanding its relevance beyond metabolic pathways.

Researchers rely on these antibodies to unravel PGAM2’s pathophysiological mechanisms, aiding diagnostics and therapeutic targeting for related disorders. Proper validation remains critical due to potential cross-reactivity with PGAM1 or other isoforms.