NDUFS3抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-6: 293T cells, NIH/3T3 cells, Raw264.7 cells, hela cells, human fetal brain  tissue, mouse brain tissue, Primary antibody: P08554(NDUFS3 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P08554(NDUFS3 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain  tissue using P08554(NDUFS3 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The NDUFS3 antibody is a crucial tool in studying mitochondrial complex I (NADH:ubiquinone oxidoreductase), a key component of the electron transport chain responsible for oxidative phosphorylation. NDUFS3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 3) is a 30 kDa nuclear-encoded subunit of complex I, located in the hydrophobic core of the membrane arm. It plays a structural and functional role in electron transfer and enzyme assembly. Antibodies targeting NDUFS3 are widely used to investigate complex I deficiencies linked to mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson's or Leigh syndrome), and cancer metabolism.

These antibodies enable researchers to detect NDUFS3 protein expression levels via Western blotting, immunofluorescence, or immunohistochemistry, aiding in the assessment of complex I integrity in cellular or tissue samples. Studies using NDUFS3 antibodies have revealed its downregulation in conditions involving impaired energy production, oxidative stress, or mitochondrial DNA mutations. Additionally, they help elucidate disease mechanisms in models of genetic mutations (e.g., in Leber's hereditary optic neuropathy) or pharmacological inhibition of complex I.

Commercial NDUFS3 antibodies are typically raised in rabbits or mice using peptide antigens corresponding to conserved regions of the human protein. Validation includes specificity testing in knockdown models or complex I-deficient samples. Their application extends to diagnostic research and therapeutic development targeting mitochondrial dysfunction.