MDH1抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 60 μg, Lane: Hela cells, Primary antibody: P08504(MDH1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

Malate dehydrogenase 1 (MDH1) is a mitochondrial enzyme that catalyzes the reversible oxidation of malate to oxaloacetate, playing a critical role in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. This enzyme is essential for cellular energy production, amino acid synthesis, and redox balance. MDH1 antibodies are immunological tools designed to detect and quantify MDH1 protein expression in various biological samples. They are widely used in research to study mitochondrial metabolism, cellular responses to metabolic stress, and diseases linked to mitochondrial dysfunction, such as cancer, neurodegenerative disorders, and metabolic syndromes.

MDH1 antibodies are commonly applied in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein localization, expression levels, and post-translational modifications. Recent studies highlight MDH1's potential role in cancer progression, where its dysregulation may influence tumor growth, metastasis, or therapeutic resistance. In neurodegenerative contexts, altered MDH1 activity has been associated with mitochondrial impairment in conditions like Alzheimer’s disease.

The development and validation of MDH1 antibodies require careful consideration of cross-reactivity with homologous proteins (e.g., MDH2) to ensure specificity. These antibodies serve as vital reagents for unraveling MDH1's functional diversity, offering insights into metabolic adaptations in health and disease.